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In more comprehensive studies, it was confirmed that, in vitro, the tandem Tudor domain of 53BP1 binds to a number of dimethylated lysine residues at the p53 C-terminus, with a preference for p53K382me2 172 , 173 The KD worth of 53BP1 Tudor-p53K382me2 is about 0.9 µM, whereas that of 53BP1 Tudor-p53K372me2 is 20 µM, indicating that the binding of 53BP1 Tudor to p53K382me2 is far stronger 173 Additional, technology of K382me2 within the p53K370R mutant retains the flexibility of p53 protein to interact with 53BP1 in vivo, suggesting that p53 K382 dimethylation is extra seemingly the primary goal of 53BP1 Tudor domains 172 Since different regions of p53 and 53BP1 additionally interact, further examine is required to outline the specificity and capabilities of p53 C-terminal lysine methylation for 53BP1 affiliation and different activities.
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